Procjena in vitro protoskolicidne učinkovitosti metanolnih biljnih ekstrakata na hidatidne ciste

The present study aimed at an evaluation of the protoscolicidal potential of methanolic extracts of three herbs; Ferula asafoetida (dried latex), Trachyspermum ammi (fruits) and Hippophae salicifolia (leaves) at three different concentrations (10, 20 and 30 mg/mL) for 20, 40 and 60 minute treatment intervals, with respect to standard allopathic drug albendazole. The in-vitro viability of the protoscolices was assessed by the Eosin exclusion method. The slope of mortality and lethal concentration for 50% (LC50) was determined from the regression graphs of the probit mortality of protoscolices plotted against log values of increasing concentrations of herbal extracts for 60 minute treatment intervals. The percentage of mortality caused by various extracts at concentrations ranging from 10-30 mg/mL varied from 29.54-97.76% with maximum mortality of 97.76, 97.30 and 81.91% recorded with methanolic extracts of F. asafoetida, T. ammi and H. salicifolia, respectively. Among all the extracts, the highest protoscolicidal activity was exhibited by F. asafoetida at 20 and 30 mg/mL concentrations in the 60 (97.16%) and 40/60 minutes (97.20% and 97.76%) treatments, respectively, and it showed a minimum LC50 value of 0.204, followed by T. ammi at 0.933. As compared to the standard allopathic drug, albendazole, the methanolic extracts of F. asafoetida and T. ammi exhibited better or comparable protoscolicidal activities.Cilj je istraživanja bila procjena protoskolicidnog potencijala metanolnih ekstrakata triju biljaka: Ferula asafoetida (sušena smola), Trachyspermum ammi (voće) i Hippophae salicifolia (lišće) u trima različitim koncentracijama (10, 20 i 30 mg/mL) u 20, 40 i 60-minutnim pokusnim intervalima u odnosu na standardni alopatski lijek albendazol. In vitro vitalnost protoskoleksa procijenjena je metodom isključivanja eozina. Krivulja pomora i smrtonosne koncentracije za 50 % (LC50) određena je iz regresijskih grafikona mortaliteta protoskoleksa u odnosu na log vrijednosti rastućih koncentracija biljnih ekstrakata tijekom 60-minutnog intervala. Pomor uzrokovan različitim ekstraktima u koncentracijama od 10 do 30 mg/mL varirao je od 29,54 do 97,76 % s najvišim postotkom od 97,76, 97,30 i 81,91 % u slučaju metanolnih ekstrakata F. asafoetida, T. ammi i H. salicifolia. Od svih primijenjenih ekstrakata najvišu protoskolicidnu aktivnost imala je F. asafoetida u koncentracijama od 20 i 30 mg/mL tijekom tretmana od 60 (97,16 %) i 40/60 minuta (97,20 i 97,76%), te je pokazala minimalnu vrijednost LC50 od 0,204, praćenu s T. ammi od 0,933. Usporedbom sa standardnim antiparazitikom albendazolom, metanolni ekstrakti biljaka F. asafoetida i T. ammi ostvarili su bolji ili slični protoskolicidni učinak